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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UROD Polyclonal antibody (15547-1-AP)

    WB analysis using 15547-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using UROD Polyclonal antibody (15547-1-AP)

    WB analysis of rat kidney using 15547-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UROD Polyclonal antibody (15547-1-AP)

    WB analysis of HepG2 using 15547-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using UROD Polyclonal antibody (15547-1-AP)

    WB analysis of HEK-293 using 15547-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using UROD Polyclonal antibody (15547-1-AP)

    IP experiment of K-562 using 15547-1-AP

    IP result of anti-UROD (IP:15547-1-AP, 4ug; Detection:15547-1-AP 1:500) with K-562 cells lysate 4400 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using UROD Polyclonal antibody (15547-1-AP)

    IHC staining of human kidney using 15547-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using UROD Polyclonal antibody (15547-1-AP)

    IHC staining of human kidney using 15547-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using UROD Polyclonal antibody (15547-1-AP)

    IF Staining of HEK-293 using 15547-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UROD antibody (15547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

UROD Polyclonal antibody

UROD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15547-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

URO-D, EC:4.1.1.37

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HepG2 cells, HEK-293 cells, rat kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15547-1-AP targets UROD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7905

Product name: Recombinant human UROD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-367 aa of BC001778

Sequence: MEANGLGPQGFPELKNDTFLRAAWGEETDYTPVWCMRQAGRYLPEFRETRAAQDFFSTCRSPEACCELTLQPLRRFPLDAAIIFSDILVVPQALGMEVTMVPGKGPSFPEPLREEQDLERLRDPEVVASELGYVFQAITLTRQRLAGRVPLIGFAGAPWTLMTYMVEGGGSSTMAQAKRWLYQRPQASHQLLRILTDALVPYLVGQVVAGAQALQLFESHAGHLGPQLFNKFALPYIRDVAKQVKARLREAGLAPVPMIIFAKDGHFALEELAQAGYEVVGLDWTVAPKKARECVGKTVTLQVNLDPCALYASEEEIGQLVKQMLDDFGPHRYIANLGHGLYPDMDPEHVGAFVDAVHKHSRLLRQN

相同性解析による交差性が予測される生物種
Full Name uroporphyrinogen decarboxylase
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC001778
Gene Symbol UROD
Gene ID (NCBI) 7389
RRIDAB_2213813
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP06132
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UROD antibody 15547-1-APDownload protocol
IHC protocol for UROD antibody 15547-1-APDownload protocol
IF protocol for UROD antibody 15547-1-APDownload protocol
IP protocol for UROD antibody 15547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.

Authors - Gokhan Unlu
View Article
mouseWB

Cell Mol Gastroenterol Hepatol

The histamine pathway is a target to treat hepatic experimental erythropoietic protoporphyria

Authors - Ning Kuo
View Article