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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using UROC1 Polyclonal antibody (25032-1-AP)

    WB analysis of mouse liver using 25032-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25032-1-AP (UROC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using UROC1 Polyclonal antibody (25032-1-AP)

    IP experiment of mouse liver using 25032-1-AP

    IP result of anti-UROC1 (IP:25032-1-AP, 4ug; Detection:25032-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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UROC1 Polyclonal antibody

UROC1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25032-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

HMFN0320, UROC1, Urocanase, urocanase domain containing 1, Urocanate hydratase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25032-1-AP targets UROC1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UROC1 fusion protein Ag18143 相同性解析による交差性が予測される生物種
Full Name urocanase domain containing 1
Calculated molecular weight 676 aa, 75 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC115405
Gene Symbol UROC1
Gene ID (NCBI) 131669
RRIDAB_2879859
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96N76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UROC1, also named as Urocanase and Imidazolonepropionate hydrolase, is involved in Urocanase deficiency (UROCD). UROC1 has some isoforms with MW 65-75 kDa and 80 kDa.

Protocols

Product Specific Protocols
WB protocol for UROC1 antibody 25032-1-APDownload protocol
IP protocol for UROC1 antibody 25032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO Mol Med

Luteolin detoxifies DEHP and prevents liver injury by degrading Uroc1 protein in mice

Authors - Huiting Wang
View Article