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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis using 15285-1-AP (same clone as 15285-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of human placenta using 15285-1-AP (same clone as 15285-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of HepG2 using 15285-1-AP (same clone as 15285-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of A549 using 15285-1-AP (same clone as 15285-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of HepG2 using 15285-1-AP (same clone as 15285-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of mouse liver using 15285-1-AP (same clone as 15285-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using URM1 Polyclonal antibody (15285-1-AP)

    WB analysis of A549 using 15285-1-AP (same clone as 15285-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using URM1 Polyclonal antibody (15285-1-AP)

    IP experiment of HepG2 using 15285-1-AP (same clone as 15285-1-PBS)

    IP result of anti-URM1 (IP:15285-1-AP, 4ug; Detection:15285-1-AP 1:500) with HepG2 cells lysate 3360 ug. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of mouse liver using 15285-1-AP (same clone as 15285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of mouse liver using 15285-1-AP (same clone as 15285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of human hepatocirrhosis using 15285-1-AP (same clone as 15285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of human hepatocirrhosis using 15285-1-AP (same clone as 15285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of human liver using 15285-1-AP (same clone as 15285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using URM1 Polyclonal antibody (15285-1-AP)

    IHC staining of human liver using 15285-1-AP (same clone as 15285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

URM1 Polyclonal antibody, PBS Only

URM1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15285-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

C9orf74, Ubiquitin-related modifier 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15285-1-PBS targets URM1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7448

Product name: Recombinant human URM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-101 aa of BC003581

Sequence: MAAPLSVEVEFGGGAELLFDGIKKHRVTLPGQEEPWDIRNLLIWIKKNLLKERPELFIQGDSVRPGILVLINDADWELLGELDYQLQDQDSVLFISTLHGG

相同性解析による交差性が予測される生物種
Full Name ubiquitin related modifier 1 homolog (S. cerevisiae)
Calculated molecular weight 11 kDa
Observed molecular weight 11 kDa
GenBank accession numberBC003581
Gene Symbol URM1
Gene ID (NCBI) 81605
RRIDAB_2213808
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTM9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Urm1 (ubiquitin related modifier 1) is a molecular fossil in the class of ubiquitin-like proteins (UBLs). It encompasses characteristics of classical UBLs, such as ubiquitin or SUMO (small ubiquitin-related modifier), but also of bacterial sulfur-carrier proteins (SCP) (PMID: 33499055). By the assistance of an E1-like activation enzyme Uba4, Urm1 can function as a modifier to target proteins, called urmylation (PMID: 33548388). The ubiquitin-like protein Urm1 can be covalently conjugated to other proteins, such as the yeast thioredoxin peroxidase protein Ahp1p, through a mechanism involving the ubiquitin E1-like enzyme Uba4 (PMID: 19047990).

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