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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using URB1-Specific Polyclonal antibody (20023-1-AP)

    WB analysis of K-562 using 20023-1-AP (same clone as 20023-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 20023-1-AP (URB1-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20023-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using URB1-Specific Polyclonal antibody (20023-1-AP)

    WB analysis of K-562 using 20023-1-AP (same clone as 20023-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 20023-1-AP (URB1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20023-1-PBS in a different storage buffer formulation.

URB1-Specific Polyclonal antibody, PBS Only

URB1-Specific Polyclonal Antibody for WB, Indirect ELISA
Cat No. 20023-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20023-1-PBS targets URB1-Specific in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name URB1 ribosome biogenesis 1 homolog (S. cerevisiae)
Calculated molecular weight 254 kDa
Observed molecular weight 240-260 kDa
GenBank accession numberNM_014825
Gene Symbol URB1
Gene ID (NCBI) 9875
RRIDAB_10667000
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60287
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

URB1, also named as C21orf108, KIAA0539 and NPA1, is a 2,271 amino acid protein that is homologous to the S. cerevisiae protein URB1 which is a nucleolar protein involved in the normal accumulation of 25S and 5.8S rRNAs and may also play a role in the biosynthesis of the 60S ribosomal unit. Also localized to the nucleolus, human URB1 is thought to have the same functions as S. cerevisiae URB1. The antibody is specific to URB1.

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