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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRQ Recombinant monoclonal antibody (87199-1-RR)

    WB analysis using 87199-1-RR (same clone as 87199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87199-1-RR (UQCRQ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87199-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UQCRQ Recombinant monoclonal antibody (87199-1-RR)

    WB analysis using 87199-1-RR (same clone as 87199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87199-1-RR (UQCRQ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using UQCRQ Recombinant monoclonal antibody (87199-1-RR)

    IHC staining of mouse heart using 87199-1-RR (same clone as 87199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87199-1-RR (UQCRQ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87199-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using UQCRQ Recombinant monoclonal antibody (87199-1-RR)

    IHC staining of rat liver using 87199-1-RR (same clone as 87199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 87199-1-RR (UQCRQ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87199-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UQCRQ Recombinant monoclonal antibody (87199-1-RR)

    IF Staining of HepG2 using 87199-1-RR (same clone as 87199-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UQCRQ antibody (87199-1-RR, Clone: 252364C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87199-1-PBS in a different storage buffer formulation.

  • Affinity of 87199-1-RR

    Affinity of 87199-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87199-1-RR against Human UQCRQ were performed. The affinity constant is 2.79 nM.

UQCRQ Recombinant monoclonal antibody, PBS Only

UQCRQ Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87199-1-PBS
Clone No.252364C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Complex III subunit 8, Complex III subunit VIII, Cytochrome b-c1 complex subunit 8, QCR8, QP C

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87199-1-PBS targets UQCRQ in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag6907

Product name: Recombinant human UQCRQ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-82 aa of BC001390

Sequence: MGREFGNLTRMRHVISYSLSPFEQRAYPHVFTKGIPNVLRRIRESFFRVVPQFVVFYLIYTWGTEEFERSKRKNPAAYENDK

相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC001390
Gene Symbol UQCRQ
Gene ID (NCBI) 27089
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO14949
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UQCRQ(Cytochrome b-c1 complex subunit 8) belongs to the UQCRQ/QCR8 family. UQCRQ is a ubiquinone-binding protein localized to the cytochrome bc1 region of the mitochondrial respiratory chain. The deduced 110-amino acid protein has a relatively hydrophobic and basic N-terminal region, an aspartic acid-rich middle region, and a glutamic acid- and lysine-rich C-terminal region.

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