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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRQ Polyclonal antibody (14975-1-AP)

    WB analysis using 14975-1-AP (same clone as 14975-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using UQCRQ Polyclonal antibody (14975-1-AP)

    WB analysis of human liver using 14975-1-AP (same clone as 14975-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using UQCRQ Polyclonal antibody (14975-1-AP)

    WB analysis of HepG2 using 14975-1-AP (same clone as 14975-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using UQCRQ Polyclonal antibody (14975-1-AP)

    IP experiment of HepG2 using 14975-1-AP (same clone as 14975-1-PBS)

    IP result of anti-UQCRQ (IP:14975-1-AP, 4ug; Detection:14975-1-AP 1:1000) with HepG2 cells lysate 3360 ug. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UQCRQ Polyclonal antibody (14975-1-AP)

    IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UQCRQ Polyclonal antibody (14975-1-AP)

    IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UQCRQ Polyclonal antibody (14975-1-AP)

    IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UQCRQ Polyclonal antibody (14975-1-AP)

    IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UQCRQ Polyclonal antibody (14975-1-AP)

    IF Staining of HepG2 using 14975-1-AP (same clone as 14975-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UQCRQ antibody (14975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

UQCRQ Polyclonal antibody, PBS Only

UQCRQ Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14975-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14975-1-PBS targets UQCRQ in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6886

Product name: Recombinant human UQCRQ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-82 aa of BC001390

Sequence: MGREFGNLTRMRHVISYSLSPFEQRAYPHVFTKGIPNVLRRIRESFFRVVPQFVVFYLIYTWGTEEFERSKRKNPAAYENDK

相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC001390
Gene Symbol UQCRQ
Gene ID (NCBI) 27089
RRIDAB_2288356
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14949
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UQCRQ(Cytochrome b-c1 complex subunit 8) belongs to the UQCRQ/QCR8 family. UQCRQ is a ubiquinone-binding protein localized to the cytochrome bc1 region of the mitochondrial respiratory chain. The deduced 110-amino acid protein has a relatively hydrophobic and basic N-terminal region, an aspartic acid-rich middle region, and a glutamic acid- and lysine-rich C-terminal region.

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