Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, A2780 cells, mouse heart tissue, rat heart tissue, human heart tissue, human placenta |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88500-1-RR targets UQCRFS1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag13315 Product name: Recombinant human UQCRFS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-274 aa of BC010035 Sequence: MLSVAARSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSGQAVRRPLVASVGLNVPASVCYSHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC010035 |
| Gene Symbol | UQCRFS1 |
| Gene ID (NCBI) | 7386 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P47985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UQCRFS1 antibody 88500-1-RR | Download protocol |
| IP protocol for UQCRFS1 antibody 88500-1-RR | Download protocol |
| WB protocol for UQCRFS1 antibody 88500-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |