Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells, mouse heart tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 28 publications below |
| IHC | See 2 publications below |
Product Information
18443-1-AP targets UQCRFS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UQCRFS1 fusion protein Ag13315 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC010035 |
| Gene Symbol | UQCRFS1 |
| Gene ID (NCBI) | 7386 |
| RRID | AB_10699875 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P47985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein. This antibody is specific to UQCRFS1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UQCRFS1 antibody 18443-1-AP | Download protocol |
| IHC protocol for UQCRFS1 antibody 18443-1-AP | Download protocol |
| IF protocol for UQCRFS1 antibody 18443-1-AP | Download protocol |
| IP protocol for UQCRFS1 antibody 18443-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. | ||
EMBO J EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer. | ||
J Cachexia Sarcopenia Muscle MyD88 deficiency ameliorates weight loss caused by intestinal oxidative injury in an autophagy-dependent mechanism. | ||
Cell Biosci Hinokitiol-iron complex is a ferroptosis inducer to inhibit triple-negative breast tumor growth | ||
J Nanobiotechnology Cur@SF NPs alleviate Friedreich's ataxia in a mouse model through synergistic iron chelation and antioxidation. | ||
Int J Mol Sci Contemporary Antiretroviral Therapy Dysregulates Iron Transport and Augments Mitochondrial Dysfunction in HIV-Infected Human Microglia and Neural-Lineage Cells |