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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UQCRC2 Monoclonal antibody (67547-1-Ig)

    WB analysis using 67547-1-Ig (same clone as 67547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67547-1-Ig (UQCRC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67547-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UQCRC2 Monoclonal antibody (67547-1-Ig)

    WB analysis using 67547-1-Ig (same clone as 67547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67547-1-Ig (UQCRC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UQCRC2 Monoclonal antibody (67547-1-Ig)

    IHC staining of human colon cancer using 67547-1-Ig (same clone as 67547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UQCRC2 Monoclonal antibody (67547-1-Ig)

    IHC staining of human colon cancer using 67547-1-Ig (same clone as 67547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UQCRC2 Monoclonal antibody (67547-1-Ig)

    IHC staining of human liver cancer using 67547-1-Ig (same clone as 67547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UQCRC2 Monoclonal antibody (67547-1-Ig)

    IHC staining of human liver cancer using 67547-1-Ig (same clone as 67547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UQCRC2 Monoclonal antibody (67547-1-Ig)

    IHC staining of human liver cancer using 67547-1-Ig (same clone as 67547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UQCRC2 Monoclonal antibody (67547-1-Ig)

    IHC staining of human liver cancer using 67547-1-Ig (same clone as 67547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67547-1-Ig (UQCRC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67547-1-PBS in a different storage buffer formulation.

UQCRC2 Monoclonal antibody, PBS Only

UQCRC2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67547-1-PBS
Clone No.1A5G11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

1A5G11, Complex III subunit 2, Core protein II, Cytochrome b-c1 complex subunit 2, mitochondrial, QCR2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67547-1-PBS targets UQCRC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6874

Product name: Recombinant human UQCRC2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 103-453 aa of BC000484

Sequence: IEAVGGKLSVTATRENMAYTVECLRGDVDILMEFLLNVTTAPEFRRWEVADLQPQLKIDKAVAFQNPQTHVIENLHAAAYRNALANPLYCPDYRIGKVTSEELHYFVQNHFTSARMALIGLGVSHPVLKQVAEQFLNMRGGLGLSGAKANYRGGEIREQNGDSLVHAAFVAESAVAGSAEANAFSVLQHVLGAGPHVKRGSNTTSHLHQAVAKATQQPFDVSAFNASYSDSGLFGIYTISQATAAGDVIKAAYNQVKTIAQGNLSNTDVQAAKNKLKAGYLMSVESSECFLEEVGSQALVAGSYMPPSTVLQQIDSVANADIINAAKKFVSGQKSMAASGNLGHTPFVDEL

相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase core protein II
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000484
Gene Symbol UQCRC2
Gene ID (NCBI) 7385
RRIDAB_2882763
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP22695
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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