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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UPK3A Polyclonal antibody (55229-1-AP)

    WB analysis using 55229-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55229-1-AP (UPK3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using UPK3A Polyclonal antibody (55229-1-AP)

    IHC staining of mouse bladder using 55229-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 55229-1-AP (Uroplakin III antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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UPK3A Polyclonal antibody

UPK3A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55229-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Uroplakin III, uroplakin 3A, UPK3, UPIIIA, UPIII

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inmouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55229-1-AP targets UPK3A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name uroplakin 3A
Calculated molecular weight 31 kDa
Observed molecular weight31-33 kDa
GenBank accession numberNM_006953
Gene Symbol UPK3A
Gene ID (NCBI) 7380
RRIDAB_3670244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75631
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin III (UPK3A) is a promising new urine marker for bladder cancer detection (PMID: 20346489). Loss of UPK3A expression is associated with clinicopathological features of invasive bladder cancer and poor prognosis in upper tract urothelial carcinoma (PMID: 18313120; PMID: 16686732). UPK3A has a calculated molecular weight of 30.7 kDa, with an apparent molecular weight of 47 kDa due to three potential N-glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for UPK3A antibody 55229-1-APDownload protocol
IHC protocol for UPK3A antibody 55229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols