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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using UPK1B Polyclonal antibody (26853-1-AP)

    IHC staining of human urothelial carcinoma using 26853-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using UPK1B Polyclonal antibody (26853-1-AP)

    IHC staining of human urothelial carcinoma using 26853-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using UPK1B Polyclonal antibody (26853-1-AP)

    IHC staining of mouse bladder using 26853-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using UPK1B Polyclonal antibody (26853-1-AP)

    IHC staining of mouse bladder using 26853-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 26853-1-AP (UPK1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UPK1B Polyclonal antibody

UPK1B Polyclonal Antibody for IHC, ELISA
Cat No. 26853-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

Tetraspanin 20, Tspan 20, TSPAN20, UP1b, UPIB, UPK1, UPK1B, uroplakin 1B, Uroplakin Ib

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman urothelial carcinoma tissue, mouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26853-1-AP targets UPK1B in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25471

Product name: Recombinant human UPK1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 108-229 aa of BC063568

Sequence: TAATQRDFFTPNLFLKQMLERYQNNSPPNNDDQWKNNGVTKTWDRLMLQDNCCGVNGPSDWQKYTSAFRTENNDADYPWPRQCCVMNNLKEPLNLEACKLGVPGFYHNQGCYELISGPMNRH

相同性解析による交差性が予測される生物種
Full Name uroplakin 1B
GenBank accession numberBC063568
Gene Symbol UPK1B
Gene ID (NCBI) 7348
RRIDAB_3085912
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75841
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1B (UPK1B) belongs to the tetraspanin (TM4SF) family. UPK1B can promote the proliferation, invasion and metastasis of tumor cells and regulate the development and progression of tumors (PMID: 30229818).

Protocols

Product Specific Protocols
IHC protocol for UPK1B antibody 26853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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