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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UPF3B Polyclonal antibody

UPF3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23301-1-AP

Synonyms

HUPF3B, hUpf3p X, MRXS14, RENT3B, UPF3B, UPF3X

Validation Data Gallery

Applications:
  • WB analysis using 23301-1-AP

    WB analysis using 23301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 23301-1-AP

    WB analysis of HEK-293 using 23301-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 23301-1-AP

    WB analysis of K-562 using 23301-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 23301-1-AP

    WB analysis of mouse brain using 23301-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 23301-1-AP

    IHC staining of human testis using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50.

  • IHC staining of human testis using 23301-1-AP

    IHC staining of human testis using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50.

  • IHC staining of human skeletal muscle using 23301-1-AP

    IHC staining of human skeletal muscle using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 23301-1-AP

    IHC staining of human skeletal muscle using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 23301-1-AP

    IHC staining of human heart using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 23301-1-AP

    IHC staining of human heart using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, K-562 cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman testis tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 23301-1-AP is UPF3B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UPF3B fusion protein Ag18568 相同性解析による交差性が予測される生物種
Full Name UPF3 regulator of nonsense transcripts homolog B (yeast)
Calculated molecular weight 483 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC121017
Gene symbol UPF3B
Gene ID (NCBI) 65109
RRIDAB_2879249
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UPF3B antibody 23301-1-APDownload protocol
IHC protocol for UPF3B antibody 23301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Genet

Molecular Characterization and Clinical Relevance of RNA Binding Proteins in Colorectal Cancer.

Authors - Zhen Zhang
View Article