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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UPF3B Polyclonal antibody (23301-1-AP)

    WB analysis using 23301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UPF3B Polyclonal antibody (23301-1-AP)

    WB analysis using 23301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using UPF3B Polyclonal antibody (23301-1-AP)

    IHC staining of human ovary tumor using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23301-1-AP (UPF3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using UPF3B Polyclonal antibody (23301-1-AP)

    IHC staining of human ovary tumor using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23301-1-AP (UPF3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using UPF3B Polyclonal antibody (23301-1-AP)

    IHC staining of human heart using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using UPF3B Polyclonal antibody (23301-1-AP)

    IHC staining of human heart using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using UPF3B Polyclonal antibody (23301-1-AP)

    IHC staining of human skeletal muscle using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using UPF3B Polyclonal antibody (23301-1-AP)

    IHC staining of human skeletal muscle using 23301-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

UPF3B Polyclonal antibody

UPF3B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23301-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RENT3B, hUpf3B, hUpf3p X, hUpf3p-X, MRXS14

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, mouse brain tissue
Positive IHC detected inhuman ovary tumor tissue, human skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

23301-1-AP targets UPF3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18568

Product name: Recombinant human UPF3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 288-470 aa of BC121017

Sequence: AKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQEHILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQESGISHRKEGGEE

相同性解析による交差性が予測される生物種
Full Name UPF3 regulator of nonsense transcripts homolog B (yeast)
Calculated molecular weight 483 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC121017
Gene Symbol UPF3B
Gene ID (NCBI) 65109
RRIDAB_2879249
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZI7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Regulator of nonsense transcripts 3B (UPF3B) is also named as RENT3B, and belongs to the RENT3 family. UPF3B is a core junction protein in the Nonsense-mediated RNA decay (NMD) pathway, involved in the exonuclear transport and quality supervision of mRNA. And UPF3B showed heightened expression in hepatocellular carcinoma (HCC) tissues and was linked with adverse prognosis (PMID: 38889220). UPF3B promotes the dissociation of post-termination ribosomal complexes that lack nascent peptide. UPF3B could promote ribosome recycling, and UPF3B serves as a direct regulator of translation termination (PMID: 29333258).

Protocols

Product Specific Protocols
IHC protocol for UPF3B antibody 23301-1-APDownload protocol
WB protocol for UPF3B antibody 23301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Genet

Molecular Characterization and Clinical Relevance of RNA Binding Proteins in Colorectal Cancer.

Authors - Zhen Zhang
View Article
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