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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UPF3A Polyclonal antibody (17114-1-AP)

    WB analysis using 17114-1-AP (same clone as 17114-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using UPF3A Polyclonal antibody (17114-1-AP)

    WB analysis of human testis using 17114-1-AP (same clone as 17114-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17114-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using UPF3A Polyclonal antibody (17114-1-AP)

    WB analysis of HeLa using 17114-1-AP (same clone as 17114-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using UPF3A Polyclonal antibody (17114-1-AP)

    IHC staining of human testis using 17114-1-AP (same clone as 17114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17114-1-AP (UPF3A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 17114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using UPF3A Polyclonal antibody (17114-1-AP)

    IHC staining of human testis using 17114-1-AP (same clone as 17114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17114-1-AP (UPF3A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 17114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using UPF3A Polyclonal antibody (17114-1-AP)

    IHC staining of human heart using 17114-1-AP (same clone as 17114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17114-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using UPF3A Polyclonal antibody (17114-1-AP)

    IHC staining of human heart using 17114-1-AP (same clone as 17114-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17114-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UPF3A Polyclonal antibody (17114-1-AP)

    IF Staining of HeLa using 17114-1-AP (same clone as 17114-1-PBS)

    Immunofluorescent analysis of HeLa cells using 17114-1-AP (UPF3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17114-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

UPF3A Polyclonal antibody, PBS Only

UPF3A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17114-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17114-1-PBS targets UPF3A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10799

Product name: Recombinant human UPF3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 93-443 aa of BC023569

Sequence: AHDYFEFFAADLSLYPHLYSRAYINFRNPDDILLFRDRFDGYIFLDSKDPEYKKFLETYCVEEEKTSANPETLLGEMEAKTRELIARRTTPLLEYIKNRKLEKQRIREEKREERRRRELEKKRLREEEKRRRREEERCKKKETDKQKKIAEKEVRIKLLKKPEKGEEPTTEKPKERGEEIDTGGGKQESCAPGAVVKARPMEGSLEEPQETSHSGSDKEHRDVERSQEQESEAQRYHVDDGRRHRAHHEPERLSRRSEDEQRWGKGPGQDRGKKGSQDSGAPGEAMERLGRAQRCDDSPAPRKERLANKDRPALQLYDPGARFRARECGGNRRICKAEGSGTGPEKREEAE

相同性解析による交差性が予測される生物種
Full Name UPF3 regulator of nonsense transcripts homolog A (yeast)
Calculated molecular weight 443 aa, 51 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC023569
Gene Symbol UPF3A
Gene ID (NCBI) 65110
RRIDAB_10694141
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1J1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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