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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUN1 Polyclonal antibody (24568-1-AP)

    WB analysis using 24568-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24568-1-AP (SUN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SUN1 Polyclonal antibody (24568-1-AP)

    WB analysis of Jurkat using 24568-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24568-1-AP (SUN1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SUN1 Polyclonal antibody (24568-1-AP)

    WB analysis of K-562 using 24568-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24568-1-AP (SUN1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using SUN1 Polyclonal antibody (24568-1-AP)

    IP experiment of Jurkat using 24568-1-AP

    IP result of anti-SUN1 (IP:24568-1-AP, 4ug; Detection:24568-1-AP 1:1000) with Jurkat cells lysate 3200ug.

SUN1 Polyclonal antibody

SUN1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 24568-1-AP
Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IP, ELISA

Synonyms

KIAA0810, Protein unc 84 homolog A, Sad1/unc 84 protein like 1, SUN1, unc 84 homolog A (C. elegans), UNC84A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, Jurkat cells, K-562 cells, HeLa cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24568-1-AP targets SUN1 in WB, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21117

Product name: Recombinant human UNC84A protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 4-257 aa of BC013613

Sequence: SRLHMYSPPQCVPENTGYTYALSSSYSSDALDFETEHKLDPVFDSPRMSRRSLRLATTACTLGDGEAVGADSGTSSAVSLKNRAARTTKQRRSTNKSAFSINHVSRQVTSSGVSHGGTVSLQDAVTRRPPVLDESWIREQTTVDHFWGLDDDGDLKGGNKAAIQGNGDVGAAAATAHNGFSCSNCSMLSERKDVLTAHPAPPGPVSRVYSRDRNQKCKSQSFKTQKKVCFPNLIFPFCKSQCLHYLSWRLKIIP

相同性解析による交差性が予測される生物種
Full Name unc-84 homolog A (C. elegans)
Calculated molecular weight 90 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC013613
Gene Symbol SUN1
Gene ID (NCBI) 23353
RRIDAB_2879612
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO94901
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IP protocol for SUN1 antibody 24568-1-APDownload protocol
WB protocol for SUN1 antibody 24568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Nuclear membrane protein SUN2 promotes replication of flaviviruses through modulating cytoskeleton reorganization mediated by NS1

Authors - Yanxia Huang
View Article
humanWB

Acta Biochim Biophys Sin (Shanghai)

SUN5, a testis-specific nuclear membrane protein, participates in recruitment and export of nuclear mRNA in spermatogenesis

Authors - Xiyi He
  • KD Validated
View Article
mouseWB

Int J Mol Sci

Electrical Stimulation Generates Induced Tumor-Suppressing Cells, Offering a Potential Option for Combatting Breast Cancer and Bone Metastasis

Authors - Changpeng Cui
  • KD Validated
View Article
humanWB,IF,IP

Nat Struct Mol Biol

DNA double-strand break-capturing nuclear envelope tubules drive DNA repair

Authors - Mitra Shokrollahi
View Article
humanWB

Adv Sci (Weinh)

Attenuated Nuclear Tension Regulates Progerin-Induced Mechanosensitive Nuclear Wrinkling and Chromatin Remodeling

Authors - Ji-Eun Park
View Article
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