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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using UNC79 Recombinant antibody (84642-1-RR)

    IHC staining of mouse brain using 84642-1-RR (same clone as 84642-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84642-1-RR (UNC79 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84642-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using UNC79 Recombinant antibody (84642-1-RR)

    IHC staining of mouse brain using 84642-1-RR (same clone as 84642-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84642-1-RR (UNC79 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84642-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using UNC79 Recombinant antibody (84642-1-RR)

    IF Staining of SH-SY5Y using 84642-1-RR (same clone as 84642-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using UNC79 antibody (84642-1-RR, Clone: 241628A7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84642-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84642-1-RR

    Affinity and Kinetic Characterization of 84642-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84642-1-RR against Human UNC79 were performed. The affinity constant is 11 nM.

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UNC79 Recombinant antibody, PBS Only

UNC79 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 84642-1-PBS
Clone No.241628A7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Protein unc-79 homolog, KIAA1409, 241628A7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84642-1-PBS targets UNC79 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UNC79 fusion protein Ag35440 相同性解析による交差性が予測される生物種
Full Name KIAA1409
Calculated molecular weight295 kDa
GenBank accession numberNM_020818.4
Gene Symbol UNC79
Gene ID (NCBI) 57578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9P2D8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UNC79 is one of the key auxiliary subunits of the NALCN channel complex. Together with NALCN, FAM155A, and UNC80, it constitutes the NALCN channel body and is essential for maintaining neuronal excitability, motor function, pain sensitivity, and circadian rhythm (PMID: 35387979).