Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 16905-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16905-1-AP (UNC13D/Munc13-4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16905-1-AP
WB result of UNC13D/Munc13-4 antibody (16905-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNC13D/Munc13-4 transfected K-562 cells.
WB analysis of human placenta using 16905-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 16905-1-AP (UNC13D/Munc13-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 16905-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16905-1-AP (UNC13D/Munc13-4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 16905-1-AP
IP result of anti-UNC13D/Munc13-4 (IP:16905-1-AP, 5ug; Detection:16905-1-AP 1:300) with Jurkat cells lysate 2400ug.
IHC staining of human thymus using 16905-1-AP
Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 16905-1-AP (UNC13D/Munc13-4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thymus using 16905-1-AP
Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 16905-1-AP (UNC13D/Munc13-4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UNC13D/Munc13-4 Polyclonal antibody

UNC13D/Munc13-4 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16905-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Synonyms

FHL3, HLH3, HPLH3, Munc13 4, Munc13-4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D, UNC13D/Munc13-4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, K-562 cells, human placenta tissue
Positive IP detected in	Jurkat cells
Positive IHC detected in	human thymus tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IF	See 2 publications below

Product Information

16905-1-AP targets UNC13D/Munc13-4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10519 Product name: Recombinant human UNC13D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-327 aa of BC067084 Sequence: MATLLSHPQQRPPFLRQAIKIRRRRVRDLQDPPPQMAPEIQPPSHHFSPEQRALLYEDALYTVLHRLGHPEPNHVTEASELLRYLQEAFHVEPEEHQQTLQRVRELEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGVPGGSPGSRHRQKAVVRHTIPEEETHRTQVITQTLNPVWDETFILEFEDITNASFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPRTETYPDRGQCHLQFQLIHKRRATSASRSQPSYTVHLHLLQQLVSHEVTQHEAGSTSWDGSL 相同性解析による交差性が予測される生物種
Full Name	unc-13 homolog D (C. elegans)
Calculated molecular weight	1090 aa, 103 kDa
Observed molecular weight	120 kDa
GenBank accession number	BC067084
Gene Symbol	UNC13D
Gene ID (NCBI)	201294
RRID	AB_2212713
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q70J99
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for UNC13D/Munc13-4 antibody 16905-1-AP	Download protocol
IP protocol for UNC13D/Munc13-4 antibody 16905-1-AP	Download protocol
WB protocol for UNC13D/Munc13-4 antibody 16905-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells Authors - Tatsuaki Kurosaki View Article
human	WB	J Exp Med Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency. Authors - Frank Cichocki View Article
human	WB	Blood Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D. Authors - Meeths Marie M View Article
human	WB	Front Immunol Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets. Authors - Donatella Galgano View Article
human	WB,IF	Mol Biol Cell A novel Munc13-4/S100A10/Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells. Authors - Tarek Chehab View Article
human	WB,IF	J Transl Med Recycling machinery of integrin coupled with focal adhesion turnover via RAB11-UNC13D-FAK axis for migration of pancreatic cancer cells Authors - Van-Thanh Duong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16905-1-AP

WB analysis of K-562 using 16905-1-AP

WB analysis of human placenta using 16905-1-AP

WB analysis using 16905-1-AP

IP experiment of Jurkat using 16905-1-AP

IHC staining of human thymus using 16905-1-AP

IHC staining of human thymus using 16905-1-AP

UNC13D/Munc13-4 Polyclonal antibody

UNC13D/Munc13-4 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells

Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.

Familial hemophagocytic lymphohistiocytosis type 3 (FHL3) caused by deep intronic mutation and inversion in UNC13D.

Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets.

A novel Munc13-4/S100A10/Annexin A2 complex promotes Weibel-Palade body exocytosis in endothelial cells.

Recycling machinery of integrin coupled with focal adhesion turnover via RAB11-UNC13D-FAK axis for migration of pancreatic cancer cells