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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UNC13B Polyclonal antibody (21839-1-AP)

    WB analysis using 21839-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21839-1-AP (UNC13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using UNC13B Polyclonal antibody (21839-1-AP)

    WB analysis using 21839-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21839-1-AP (UNC13B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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UNC13B Polyclonal antibody

UNC13B Polyclonal Antibody for WB, ELISA
Cat No. 21839-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

unc-13 homolog B , Protein unc-13 homolog B, Munc13-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells, SH-SY5Y cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21839-1-AP targets UNC13B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UNC13B fusion protein Ag16478 相同性解析による交差性が予測される生物種
Full Name unc-13 homolog B (C. elegans)
Calculated molecular weight 1610 aa, 183 kDa
Observed molecular weight180 kDa
GenBank accession numberBC111781
Gene Symbol UNC13B
Gene ID (NCBI) 10497
RRIDAB_3669376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14795
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UNC13A is a member of the unliganded-13 (UNC13) family and plays a crucial role in synaptic vesicle priming and exocytosis. UNC13B has been implicated in autism spectrum disorder (ASD), partial epilepsy, and schizophrenia (PMID: 38188011).

Protocols

Product Specific Protocols
WB protocol for UNC13B antibody 21839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols