Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, HeLa cells, SH-SY5Y cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21839-1-AP targets UNC13B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16478 Product name: Recombinant human UNC13B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC111781 Sequence: MSLLCVRVKRAKFQGSPDKFNTYVTLKVQNVKSTTVAVRGDQPSWEQDFMFEISRLDLGLSVEVWNKGLIWDTMVGTVWIALKTIRQSDEEGPGEWSTLEAETLMKDDEICGTRNPTPHKILLDTRFELPFDIPEEEARYWTYKWEQINALGADNEYSSQEESQRKPLPTAAAQCSFEDPDSAVDDRDSDYRSETSNSFPPPYHTASQPNASVHQFPVPVRSPQQLLLQGSSRDSCNDSMQSYDLDYPERRAISPTSSSRYGSSCNVSQGSSQLSELDQYHEQDDDHRETDSIHSCHSSHSLSRDGQAGFGEQEKPLEVTGQAEKEAACE 相同性解析による交差性が予測される生物種 |
| Full Name | unc-13 homolog B (C. elegans) |
| Calculated molecular weight | 1610 aa, 183 kDa |
| Observed molecular weight | 180 kDa |
| GenBank accession number | BC111781 |
| Gene Symbol | UNC13B |
| Gene ID (NCBI) | 10497 |
| RRID | AB_3669376 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14795 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UNC13A is a member of the unliganded-13 (UNC13) family and plays a crucial role in synaptic vesicle priming and exocytosis. UNC13B has been implicated in autism spectrum disorder (ASD), partial epilepsy, and schizophrenia (PMID: 38188011).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UNC13B antibody 21839-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |