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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UNC13A Recombinant antibody (83000-5-RR)

    WB analysis using 83000-5-RR (same clone as 83000-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83000-5-RR (UNC13A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using UNC13A Recombinant antibody (83000-5-RR)

    IP experiment of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

    IP result of anti-UNC13A (IP:83000-5-RR, 4ug; Detection:83000-5-RR 1:1000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using UNC13A Recombinant antibody (83000-5-RR)

    IHC staining of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83000-5-RR (UNC13A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using UNC13A Recombinant antibody (83000-5-RR)

    IHC staining of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83000-5-RR (UNC13A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

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UNC13A Recombinant antibody, PBS Only

UNC13A Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 83000-5-PBS
Clone No.240017A6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83000-5-PBS targets UNC13A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name unc-13 homolog A (C. elegans)
Calculated molecular weight 193 kDa
Observed molecular weight193-210 kDa
GenBank accession numberNM_001080421
Gene Symbol UNC13A
Gene ID (NCBI) 23025
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UPW8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.