Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26535-1-AP targets UNC119B in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | UNC119B fusion protein Ag24916 相同性解析による交差性が予測される生物種 |
| Full Name | unc-119 homolog B (C. elegans) |
| Observed molecular weight | 28-32 kDa |
| GenBank accession number | BC004815 |
| Gene Symbol | UNC119B |
| Gene ID (NCBI) | 84747 |
| RRID | AB_3669546 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A6NIH7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UNC119b is a myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. The myristoyl-binding activity of UNC119b is required to localize NPHP3 to the primary cilium (PMID: 22085962). Mouse Unc119b shares 90% sequence identity with human UNC119b protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UNC119B antibody 26535-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |