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UMP/CMP kinase Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-100 ng/mL

Sandwich ELISA: 39.1-2500 pg/mL

Cat no : MP02460-1

Print datasheet

Synonyms

CMPK1, CK, CMK, CMPK, Cytidine monophosphate kinase

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02460-1, Human UMP/CMP kinase Recombinant Matched Antibody Pair - PBS only. 86475-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1920. 86475-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

    Sandwich ELISA standard curve of MP02460-1

    Sandwich ELISA standard curve of MP02460-1, Human UMP/CMP kinase Recombinant Matched Antibody Pair - PBS only. 86475-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1920. 86475-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • The mean UMP/CMP kinase concentration was determined to be 383.79 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 348.74 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

    Sandwich ELISA sample test of MP02460-1

    The mean UMP/CMP kinase concentration was determined to be 383.79 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 348.74 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02460-1, UMP/CMP kinase Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86475-2-PBS. Detection antibody: 86475-1-PBS. Standard: Ag1920. Range: 0.781-100 ng/mL.

    Cytometric bead array standard curve of MP02460-1

    Cytometric bead array standard curve of MP02460-1, UMP/CMP kinase Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86475-2-PBS. Detection antibody: 86475-1-PBS. Standard: Ag1920. Range: 0.781-100 ng/mL.

  • The mean UMP/CMP kinase concentration was determined to be 134.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

    Cytometric bead array sample test of MP02460-1

    The mean UMP/CMP kinase concentration was determined to be 134.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02460-1 targets UMP/CMP kinase in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-100 ng/mL (Cytometric Bead Array); 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: CMPK1 Recombinant monoclonal antibody, PBS Only (Capture) 86475-2-PBS (251233H8). 100 μg. Concentration 1 mg/ml.

Detection antibody: UMP/CMP kinase Recombinant monoclonal antibody, PBS Only (Detector) 86475-1-PBS (251233D3). 100 μg. Concentration 1 mg/ml.

Alternative UMP/CMP kinase matched antibody pairs: MP51541-1

Matched pair protein standard: Ag1920

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86475-2-PBS
Tested Reactivity human
Immunogen CMPK1 fusion protein Ag1920
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
Synonyms UMP/CMP kinase, CK, CMK, CMPK, Cytidine monophosphate kinase
GenBank accession numberBC014961
Gene symbol CMPK1
UniprotP30085
Gene ID (NCBI) 51727
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86475-1-PBS
Tested Reactivity human
Immunogen UMP/CMP kinase fusion protein Ag1920
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
Synonyms CMPK1, CK, CMK, CMPK, Cytidine monophosphate kinase
GenBank accession numberBC014961
Gene symbol CMPK1
UniprotP30085
Gene ID (NCBI) 51727
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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