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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ULK1 Monoclonal antibody (68445-1-Ig)

    WB analysis of PC-3 using 68445-1-Ig (same clone as 68445-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ULK1 Monoclonal antibody (68445-1-Ig)

    WB analysis of HeLa using 68445-1-Ig (same clone as 68445-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ULK1 Monoclonal antibody (68445-1-Ig)

    WB analysis of Jurkat using 68445-1-Ig (same clone as 68445-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ULK1 Monoclonal antibody (68445-1-Ig)

    WB analysis using 68445-1-Ig (same clone as 68445-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ULK1 Monoclonal antibody (68445-1-Ig)

    WB analysis using 68445-1-Ig (same clone as 68445-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ULK1 Monoclonal antibody (68445-1-Ig)

    IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ULK1 Monoclonal antibody (68445-1-Ig)

    IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ULK1 Monoclonal antibody (68445-1-Ig)

    IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ULK1 Monoclonal antibody (68445-1-Ig)

    IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

ULK1 Monoclonal antibody, PBS Only

ULK1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68445-1-PBS
Clone No.1D7G1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

1D7G1, ATG1, Autophagy-related protein 1 homolog, EC:2.7.11.1, hATG1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68445-1-PBS targets ULK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag22397

Product name: Recombinant human ULK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 17-466 aa of NM_003565

Sequence: SRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSG

相同性解析による交差性が予測される生物種
Full Name unc-51-like kinase 1 (C. elegans)
Observed molecular weight 130-150 kDa
GenBank accession numberNM_003565
Gene Symbol ULK1
Gene ID (NCBI) 8408
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO75385
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

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