• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ULK1 Polyclonal antibody (27352-1-AP)

    WB analysis using 27352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ULK1 Polyclonal antibody (27352-1-AP)

    WB analysis using 27352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ULK1 Polyclonal antibody (27352-1-AP)

    WB analysis using 27352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27352-1-AP (ULK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of mouse skeletal muscle using 27352-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of human liver cancer using 27352-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of mouse skeletal muscle using 27352-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ULK1 Polyclonal antibody (27352-1-AP)

    IHC staining of human liver cancer using 27352-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27352-1-AP (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ULK1 Polyclonal antibody (27352-1-AP)

    IF Staining of HeLa using 27352-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ULK1 antibody (27352-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

ULK1 Polyclonal antibody

ULK1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27352-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Serine/threonine-protein kinase ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, C2C12 cells, HepG2 cells, HSC-T6 cellls
Positive IHC detected inmouse skeletal muscle tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27352-1-AP targets ULK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22397

Product name: Recombinant human ULK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 17-466 aa of NM_003565

Sequence: SRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSG

相同性解析による交差性が予測される生物種
Full Name unc-51-like kinase 1 (C. elegans)
Observed molecular weight 140-150 kDa
GenBank accession numberNM_003565
Gene Symbol ULK1
Gene ID (NCBI) 8408
RRIDAB_3085951
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75385
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318). ULK1 is a 113 kDa protein that it also can be detected a band of 150 kDa through the western blot (PMID: 32904616, PMID: 32899862, PMID: 37270528).

Protocols

Product Specific Protocols
WB protocol for ULK1 antibody 27352-1-APDownload protocol
IHC protocol for ULK1 antibody 27352-1-APDownload protocol
IF protocol for ULK1 antibody 27352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Carcinog

Zinc Finger Protein 263 Augments Autophagy and Promotes Intrahepatic Cholangiocarcinoma Proliferation

Authors - Zaihua Yan
View Article
humanWB

Bone Res

IGF2BP3-mediated m6A modification of RASGRF1 promoting joint injury in rheumatoid arthritis

Authors - Qishun Geng
View Article