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Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using UGT8 Polyclonal antibody (17982-1-AP)

    WB analysis of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using UGT8 Polyclonal antibody (17982-1-AP)

    WB analysis of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using UGT8 Polyclonal antibody (17982-1-AP)

    IP experiment of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

    IP result of anti-UGT8 (IP:17982-1-AP, 4ug; Detection:17982-1-AP 1:1000) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using UGT8 Polyclonal antibody (17982-1-AP)

    IHC staining of human brain using 17982-1-AP (same clone as 17982-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using UGT8 Polyclonal antibody (17982-1-AP)

    IHC staining of human brain using 17982-1-AP (same clone as 17982-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UGT8 Polyclonal antibody (17982-1-AP)

    IHC staining of human breast cancer using 17982-1-AP (same clone as 17982-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17982-1-AP (UGT8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UGT8 Polyclonal antibody (17982-1-AP)

    IHC staining of human breast cancer using 17982-1-AP (same clone as 17982-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17982-1-AP (UGT8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

UGT8 Polyclonal antibody, PBS Only

UGT8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17982-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

UGT 8, UDP-galactose-ceramide galactosyltransferase, EC:2.4.1.47, CGT, Ceramide UDP-galactosyltransferase

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17982-1-PBS targets UGT8 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12393

Product name: Recombinant human UGT8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 38-402 aa of BC075069

Sequence: FKTLASALHERGHHTVFLLSEGRDIAPSNHYSLQRYPGIFNSTTSDAFLQSKMRNIFSGRLTAIELFDILDHYTKNCDLMVGNHALIQGLKKEKFDLLLVDPNDMCGFVIAHLLGVKYAVFSTGLWYPAEVGAPAPLAYVPEFNSLLTDRMNLLQRMKNTGVYLISRLGVSFLVLPKYERIMQKYNLLPEKSMYDLVHGSSLWMLCTDVALEFPRPTLPNVVYVGGILTKPASPLPEDLQRWVNGANEHGFVLVSFGAGVKYLSEDIANKLAGALGRLPQKVIWRFSGPKPKNLGNNTKLIEWLPQNDLLGHSKIKAFLSHGGLNSIFETMYHGVPVVGIPLFGDHYDTMTRVQAKGMGILLEWK

相同性解析による交差性が予測される生物種
Full Name UDP glycosyltransferase 8
Calculated molecular weight 541 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC075069
Gene Symbol UGT8
Gene ID (NCBI) 7368
RRIDAB_2214254
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16880
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UGT8, also known as galacotosylceramide synthase or CGT (ceramide galactosyltransferase), is a key enzyme for galacotosylceramide (GalCer) biosynthesis. It is an ER transmembrane protein and has limited tissue distribution, with predominance in Schwann cells, oligodendrocytes, kidneys, testes, and intestine. UGT8 is highly enriched in myelin in the central nervous system. Its expression levels are strongly associated with histological typing in human oligodendrogliomas and astrocytomas; can be used as molecular marker to distinguish these tumors. Higher UGT8 levels had also been linked to metastatic properties of cancer cells. This antibody specifically recognizes the endogenous UGT8.

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