Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
16663-1-AP targets UGT1A9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10139 Product name: Recombinant human UGT1A9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 33-377 aa of BC058844 Sequence: MDGSHWFTMRSVVEKLILRGHEVVVVMPEVSWQLGRSLNCTVKTYSTSYTLEDLDREFKAFAHAQWKAQVRSIYSLLMGSYNDIFDLFFSNCRSLFKDKKLVEYLKESSFDAVFLDPFDNCGLIVAKYFSLPSVVFARGILCHYLEEGAQCPAPLSYVPRILLGFSDAMTFKERVRNHIMHLEEHLLCHRFFKNALEIASEILQTPVTEYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYE 相同性解析による交差性が予測される生物種 |
| Full Name | UDP glucuronosyltransferase 1 family, polypeptide A9 |
| Calculated molecular weight | 530 aa, 60 kDa |
| Observed molecular weight | 60-70 kDa |
| GenBank accession number | BC058844 |
| Gene Symbol | UGT1A9 |
| Gene ID (NCBI) | 54600 |
| RRID | AB_3669254 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60656 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UDP-glucuronosyltransferase 1A9 (UGT1A9) is an enzyme that plays a crucial role in the glucuronidation pathway. It transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. UGT1A9 is part of a complex locus that encodes several UDP-glucuronosyltransferases. The enzyme is active on phenols and is involved in the metabolism of various drugs and endogenous compounds (PMID: 15284532; 12181437; 18004212).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UGT1A9 antibody 16663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Int 4,5-Dimethoxycanthin-6-one is a novel LSD1 inhibitor that inhibits proliferation of glioblastoma cells and induces apoptosis and pyroptosis. | ||
Biochem Pharmacol Sleep deprivation alters hepatic UGT1A9 and propofol metabolism in mice |