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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using UGP2 Recombinant monoclonal antibody (86699-1-RR)

    WB analysis of HepG2 using 86699-1-RR (same clone as 86699-1-PBS)

    WB result of UGP2 antibody (86699-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UGP2 transfected HepG2 cells. This data was developed using the same antibody clone with 86699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UGP2 Recombinant monoclonal antibody (86699-1-RR)

    WB analysis using 86699-1-RR (same clone as 86699-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86699-1-RR (UGP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86699-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UGP2 Recombinant monoclonal antibody (86699-1-RR)

    IF Staining of HepG2 using 86699-1-RR (same clone as 86699-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UGP2 antibody (86699-1-RR, Clone: 251615C1 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86699-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86699-1-RR

    Affinity and Kinetic Characterization of 86699-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86699-1-RR against Human UGP2 were performed. The affinity constant is 0.979 nM.

  • Featured Product
  • KD/KO Validated

UGP2 Recombinant monoclonal antibody, PBS Only

UGP2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86699-1-PBS
Clone No.251615C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:2.7.7.9, UDPG, UDP-glucose pyrophosphorylase, UDPGP2, UGPase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86699-1-PBS targets UGP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0590

Product name: Recombinant human UGP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-288 aa of BC002954

Sequence: SQDGASQFQEVIRQELELSVKKELEKILTTASSHEFEHTKKDLDGFRKLFHRFLQEKGPSVDWGKIQRPPEDSIQPYEKIKARGLPDNISSVLNKLVVVKLNGGLGTSMGCKGPKSLIGVRNENTFLDLTVQQIEHLNKTYNTDVPLVLMNSFNTDEDTKKILQKYNHCRVKIYTFNQSRYPRINKESLLPVAKDVSYSGENTEAWYPPGHGDIYASFYNSGLLDTFIGEGKEYIFVSNIDNLGATVDLYILNHLMNPPNGKRCEFVMEVTNKTRADVKGGTLTQYE

相同性解析による交差性が予測される生物種
Full Name UDP-glucose pyrophosphorylase 2
Calculated molecular weight 56 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC002954
Gene Symbol UGP2
Gene ID (NCBI) 7360
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16851
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UDP-glucose pyrophosphorylase 2 (UGP2, synonyms: UDPG, UGPP2, UDPGP2, Phc379)is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. So far two isoforms are encoded by two transcript variants have been found.

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