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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UGDH Monoclonal antibody (67360-1-Ig)

    WB analysis using 67360-1-Ig (same clone as 67360-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67360-1-Ig (UGDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using UGDH Monoclonal antibody (67360-1-Ig)

    WB analysis of rat brain using 67360-1-Ig (same clone as 67360-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67360-1-Ig (UGDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UGDH Monoclonal antibody (67360-1-Ig)

    IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UGDH Monoclonal antibody (67360-1-Ig)

    IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UGDH Monoclonal antibody (67360-1-Ig)

    IF Staining of HepG2 using 67360-1-Ig (same clone as 67360-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UGDH antibody (67360-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

UGDH Monoclonal antibody, PBS Only

UGDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67360-1-PBS
Clone No.1E2E1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

UDP-GlcDH, UDP-Glc dehydrogenase, UDPGDH, GDH, EC:1.1.1.22

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67360-1-PBS targets UGDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29747

Product name: Recombinant human UGDH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-301 aa of BC022781

Sequence: MFEIKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGDETPEGQRAVQALCAVYEHWVPREKILTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQ

相同性解析による交差性が予測される生物種
Full Name UDP-glucose dehydrogenase
Calculated molecular weight 494 aa, 55 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC022781
Gene Symbol UGDH
Gene ID (NCBI) 7358
RRIDAB_2882612
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO60701
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).

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