Validation Data Gallery

Filter: AllWBIPIHCIF-PIF-Fro

WB analysis of A375 using 12869-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 12869-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 12869-1-AP
U-251 cells were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12869-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12869-1-AP (UGCG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A375 using 12869-1-AP
IP result of anti-UGCG (IP:12869-1-AP, 4ug; Detection:12869-1-AP 1:500) with A375 cells lysate 3200ug.
IHC staining of human lung cancer using 12869-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12869-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12869-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12869-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12869-1-AP (UGCG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 12869-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 12869-1-AP (UGCG antibody) at dilution of 1:100 (under 40x lens).
IF Staining of mouse kidney using 12869-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 12869-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 12869-1-AP
Immunofluorescent analysis of frozen mouse kidney tissue fixed with 4% PFA for 24 hours, embedded in OCT, permeabilized with 0.5% Triton X-100 PBS and subjected to antigen retrieval in sodium citrate buffer (pH 6.0) using UGCG antibody (12869-1-AP) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

UGCG Polyclonal antibody

UGCG Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Cat No. 12869-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Synonyms

Glycosylceramide synthase, Glucosylceramide synthase, GLCT-1, GCS, EC:2.4.1.80

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A375 cells, C6 cells, mouse brain tissue, U-251 cells
Positive IP detected in	A375 cells
Positive IHC detected in	human lung cancer tissue, human breast cancer tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue, mouse brain tissue
Positive IF-Fro detected in	mouse kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 10 publications below
IHC	See 4 publications below
IF	See 5 publications below

Product Information

12869-1-AP targets UGCG in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3530 Product name: Recombinant human UGCG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-312 aa of BC038711 Sequence: IYTRLHLNKKATDKQPYSKLPGVSLLKPLKGVDPNLINNLETFFELDYPKYEVLLCVQDHDDPAIDVCKKLLGKYPNVDARLFIGGKKVGINPKINNLMPGYEVAKYDLIWICDSGIRVIPDTLTDMVNQMTEKVGLVHGLPYVADRQGFAATLEQVYFGTSHPRYYISANVTGFKCVTGMSCLMRKDVLDQAGGLIAFAQYIAEDYFMAKAIADRGWRFAMSTQVAMQNSGSYSISQFQSRMIRWTKLRINMLPATIICEPISECFVASLIIGWAAHHVFR 相同性解析による交差性が予測される生物種
Full Name	UDP-glucose ceramide glucosyltransferase
Calculated molecular weight	394 aa, 45 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC038711
Gene Symbol	UGCG
Gene ID (NCBI)	7357
RRID	AB_10915469
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16739
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

UGCG, also named as GCS and GLCT-1, belongs to the glycosyltransferase 2 family. It catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. UGCG may also serve as a "flippase". It is up-regulated at the transcriptional level during keratinocyte differentiation. UGCG is a membrane protein. The calculated MW is 45 kDa. In SDS-PAGE, it is about 50-55 kDa.

Protocols

Product Specific Protocols
IF protocol for UGCG antibody 12869-1-AP	Download protocol
IHC protocol for UGCG antibody 12869-1-AP	Download protocol
IP protocol for UGCG antibody 12869-1-AP	Download protocol
WB protocol for UGCG antibody 12869-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids Authors - Junchen Liu View Article
human,mouse	WB	Cancer Res Aneuploid cell survival relies upon sphingolipid homeostasis. Authors - Yun-Chi Tang KD Validated View Article
mouse	WB,IF	Cell Mol Biol Lett UGCG modulates heart hypertrophy through B4GalT5-mediated mitochondrial oxidative stress and the ERK signaling pathway Authors - Shengyu Cui View Article
human, mouse	WB,IHC	Hum Mol Genet Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase. Authors - Alexandre Henriques View Article
human	WB,IHC	Aging (Albany NY) Identification of long-term survival-associated gene in breast cancer. Authors - Shipeng Ning View Article
human	IF	iScience Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation. Authors - Zhiqiang Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A375 using 12869-1-AP

WB analysis of C6 using 12869-1-AP

WB analysis of U-251 using 12869-1-AP

WB analysis of mouse brain using 12869-1-AP

IP experiment of A375 using 12869-1-AP

IHC staining of human lung cancer using 12869-1-AP

IHC staining of human lung cancer using 12869-1-AP

IHC staining of human breast cancer using 12869-1-AP

IHC staining of human breast cancer using 12869-1-AP

IHC staining of human lymphoma using 12869-1-AP

IF Staining of mouse kidney using 12869-1-AP

IF Staining of mouse brain using 12869-1-AP

IF Staining of mouse kidney using 12869-1-AP

UGCG Polyclonal antibody

UGCG Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Glycolysis regulates KRAS plasma membrane localization and function through defined glycosphingolipids

Aneuploid cell survival relies upon sphingolipid homeostasis.

UGCG modulates heart hypertrophy through B4GalT5-mediated mitochondrial oxidative stress and the ERK signaling pathway

Amyotrophic lateral sclerosis and denervation alter sphingolipids and up-regulate glucosylceramide synthase.

Identification of long-term survival-associated gene in breast cancer.

Liver sphingomyelin synthase 1 deficiency causes steatosis, steatohepatitis, fibrosis, and tumorigenesis: An effect of glucosylceramide accumulation.