Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, LNCaP cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14655-1-AP targets UFSP1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6256 Product name: Recombinant human UFSP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC067904 Sequence: MGDKPPGFRGSRDWIGCVEASLCLAHFGGPQGRLCHVPRGVGLHGEVERLYSHFAGGGGPVMVGGDADARSKALLGVCVGSGTEAYVLVLDPHYWGTPKSPSELQAAGWVGWQEVSAAFDPNSFYNLCLTSLSSQQQQRTLD 相同性解析による交差性が予測される生物種 |
| Full Name | UFM1-specific peptidase 1 (non-functional) |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 23 kDa |
| GenBank accession number | BC067904 |
| Gene Symbol | UFSP1 |
| Gene ID (NCBI) | 402682 |
| RRID | AB_3669192 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6NVU6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UFSP1 is the Thiol-dependent isopeptidase that specifically mediate the processing of UFM1 precursors as well as the deconjugation of UFM1 from target proteins (PMID: 35525273; 35926457).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UFSP1 antibody 14655-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
