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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using UFM1 Polyclonal antibody (15883-1-AP)

    WB analysis of mouse lung using 15883-1-AP (same clone as 15883-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15883-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UFM1 Polyclonal antibody (15883-1-AP)

    WB analysis using 15883-1-AP (same clone as 15883-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15883-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using UFM1 Polyclonal antibody (15883-1-AP)

    WB analysis of HEK-293 using 15883-1-AP (same clone as 15883-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15883-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using UFM1 Polyclonal antibody (15883-1-AP)

    WB analysis of HepG2 using 15883-1-AP (same clone as 15883-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15883-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using UFM1 Polyclonal antibody (15883-1-AP)

    WB analysis of mouse liver using 15883-1-AP (same clone as 15883-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UFM1 Polyclonal antibody (15883-1-AP)

    IHC staining of human lung cancer using 15883-1-AP (same clone as 15883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15883-1-AP (UFM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UFM1 Polyclonal antibody (15883-1-AP)

    IHC staining of human lung cancer using 15883-1-AP (same clone as 15883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15883-1-AP (UFM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15883-1-PBS in a different storage buffer formulation.

UFM1 Polyclonal antibody, PBS Only

UFM1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15883-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Ubiquitin-fold modifier 1, ubiquitin fold modifier 1, C13orf20, BM-002, BM 002

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15883-1-PBS targets UFM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8667

Product name: Recombinant human UFM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-85 aa of BC005193

Sequence: MSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAAEEFKVPAATSAIITNDGIGINPAQTAGNVFLKHGSELRIIPRDRVGSC

相同性解析による交差性が予測される生物種
Full Name ubiquitin-fold modifier 1
Calculated molecular weight 85 aa, 9 kDa
Observed molecular weight 9-12 kDa
GenBank accession numberBC005193
Gene Symbol UFM1
Gene ID (NCBI) 51569
RRIDAB_2878195
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP61960
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UFM1 (Ubiquitin-fold modifier 1) is a ubiquitin-like protein covalently conjugated with intracellular proteins through UFMylation (modification by UFM1), a process similar to ubiquitylation (PMID: 38141606). UFM1 is conjugated to its target proteins by E1-like activating enzyme UBE1DC1 and E2-like conjugating enzyme UFC1 in a manner analogous to ubiquitylation (PMID: 28234446; 33066455). At the molecular level, UFMylation is an important mediator of the protein function. Dysregulation of the UFM1 system, e.g., the knockout of UFMylation components, disturbs proteome homeostasis and triggers endoplasmic reticulum stress. Such changes are linked to developmental disorders, tumorigenesis, tissue injury, inflammation, and several hereditary neurological syndromes (PMID: 36932998). Western blot images showed that bands larger than 100kd may be proteins modified by UFM1.

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