Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, rat spleen tissue, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 14 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
| IP | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
12384-1-AP targets UCHL3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3053 Product name: Recombinant human UCHL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-230 aa of BC018125 Sequence: MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERARYLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGETSDETLLEDAIEVCKKFMERDPDELRFNAIALSAA 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC018125 |
| Gene Symbol | UCHL3 |
| Gene ID (NCBI) | 7347 |
| RRID | AB_2210915 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15374 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UCHL3 antibody 12384-1-AP | Download protocol |
| IP protocol for UCHL3 antibody 12384-1-AP | Download protocol |
| WB protocol for UCHL3 antibody 12384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer
| ||
Nat Commun Structural basis for specific inhibition of the deubiquitinase UCHL1
| ||
Genes Dev A phosphorylation-deubiquitination cascade regulates the BRCA2-RAD51 axis in homologous recombination.
| ||
Oncogene GSK3β and UCHL3 govern RIPK4 homeostasis via deubiquitination to enhance tumor metastasis in ovarian cancer | ||
Am J Cancer Res UCHL3 promotes pancreatic cancer progression and chemo-resistance through FOXM1 stabilization.
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