Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, RT-4 cells, U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32722-1-AP targets UBXN7 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38516 Product name: Recombinant human UBXN7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-200 aa of NM_015562.1 Sequence: LDGGGIAEEPSTSSASVSTVRPHTEEEVRAPIPQKQEILVEPEPLFGAPKRRRPARSIFDGFRDFQTETIRQEQELRNGGAIDKKLTTLADLFRPPIDLMHKGSFETAKECGQMQNKWLMINIQNVQDFACQCLNRDVWSNEAVKNIIREH 相同性解析による交差性が予測される生物種 |
| Full Name | UBX domain protein 7 |
| Calculated molecular weight | 55kDa，489aa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | NM_015562.1 |
| Gene Symbol | UBXN7 |
| Gene ID (NCBI) | 26043 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O94888 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBXN7(UBX domain protein 7) is a protein with ubiquitin-binding activity and ubiquitin-proteasome-binding activity. It contains four different domains, namely, the N-terminal UBA domain, the UAS domain, the Ubiquitin Interaction motif (UIM) and the C-terminal UBX domain. The level of UBXN7 will affect the metabolic state of cells. High levels of UBXN7 and HIF-1α accumulation support glycolysis, while inactivation of UBXN7 and activation of NRF2 increase oxidative phosphorylation (PMID: 33444648).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBXN7 antibody 32722-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |