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  • Featured Product
  • KD/KO Validated

UBXD1 Polyclonal antibody

UBXD1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14706-1-AP

Synonyms

UBX domain protein 6, UBXD1, UBXDC2, UBXN6

Validation Data Gallery

Applications:
  • WB analysis of PC-3 using 14706-1-AP

    WB analysis of PC-3 using 14706-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 14706-1-AP

    WB analysis of mouse brain using 14706-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14706-1-AP

    WB analysis of HeLa using 14706-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 14706-1-AP

    IHC staining of mouse testis using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 14706-1-AP

    IHC staining of mouse testis using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 14706-1-AP

    IHC staining of human kidney using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 14706-1-AP

    IHC staining of human kidney using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human placenta using 14706-1-AP

    IHC staining of human placenta using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human placenta using 14706-1-AP

    IHC staining of human placenta using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human testis using 14706-1-AP

    IHC staining of human testis using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 14706-1-AP

    IHC staining of human testis using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human skin using 14706-1-AP

    IHC staining of human skin using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 14706-1-AP

    IHC staining of human skin using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human brain using 14706-1-AP

    IHC staining of human brain using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 14706-1-AP

    IHC staining of human brain using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human spleen using 14706-1-AP

    IHC staining of human spleen using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 14706-1-AP

    IHC staining of human spleen using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human lung using 14706-1-AP

    IHC staining of human lung using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 14706-1-AP

    IHC staining of human lung using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human ovary using 14706-1-AP

    IHC staining of human ovary using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human ovary using 14706-1-AP

    IHC staining of human ovary using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of mouse brain using 14706-1-AP

    IHC staining of mouse brain using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 14706-1-AP

    IHC staining of mouse brain using 14706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14706-1-AP

    IF Staining of HeLa using 14706-1-AP

    Immunofluorescent analysis of Hela cells, using UBXN6 antibody 14706-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IF Staining of HeLa using 14706-1-AP

    IF Staining of HeLa using 14706-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14706-1-AP (UBXD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, mouse brain tissue
Positive IHC detected inmouse testis tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, mouse brain tissue, human spleen tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14706-1-AP targets UBXD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBXD1 fusion protein Ag6394 相同性解析による交差性が予測される生物種
Full Name UBX domain protein 6
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC008288
Gene symbol UBXN6
Gene ID (NCBI) 80700
RRIDAB_2210488
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBXD1 antibody 14706-1-APDownload protocol
IHC protocol for UBXD1 antibody 14706-1-APDownload protocol
IF protocol for UBXD1 antibody 14706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Chem Biol

Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.

Authors - Xiaojun Wu
  • KD Validated
View Article
humanIF

Acta Neuropathol Commun

Proximity proteomics of C9orf72 dipeptide repeat proteins identifies molecular chaperones as modifiers of poly-GA aggregation.

Authors - Feilin Liu
View Article
humanWB

J Immunol

UBX Domain Protein 6 Positively Regulates JAK-STAT1/2 Signaling.

Authors - Harshada Ketkar
View Article
humanIF

J Cell Sci

The p97-UBXN1 complex regulates aggresome formation.

Authors - Sirisha Mukkavalli
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tingting (Verified Customer) (01-16-2020)

Very clear bands

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Trophoblast cell, HEK293T