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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBXD2 Polyclonal antibody (21052-1-AP)

    WB analysis using 21052-1-AP (same clone as 21052-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21052-1-AP (UBXD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21052-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using UBXD2 Polyclonal antibody (21052-1-AP)

    IP experiment of mouse brain using 21052-1-AP (same clone as 21052-1-PBS)

    IP result of anti-UBXD2 (IP:21052-1-AP, 4ug; Detection:21052-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 21052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using UBXD2 Polyclonal antibody (21052-1-AP)

    IHC staining of human colon cancer using 21052-1-AP (same clone as 21052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21052-1-AP (UBXD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21052-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using UBXD2 Polyclonal antibody (21052-1-AP)

    IF Staining of HEK-293 using 21052-1-AP (same clone as 21052-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UBXD2 antibody (21052-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21052-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using UBXD2 Polyclonal antibody (21052-1-AP)

    IF Staining of HEK-293 using 21052-1-AP (same clone as 21052-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UBXD2 antibody (21052-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21052-1-PBS in a different storage buffer formulation.

UBXD2 Polyclonal antibody, PBS Only

UBXD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21052-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21052-1-PBS targets UBXD2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13440

Product name: Recombinant human UBXN4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 160-508 aa of BC035594

Sequence: TSDTKSDTATGGESAGHATSSQEPSGCSDQRPAEDLNIRVERLTKKLEERREEKRKEEEQREIKKEIERRKTGKEMLDYKRKQEEELTKRMLEERNREKAEDRAARERIKQQIALDRAERAARFAKTKEEVEAAKAAALLAKQAEMEVKRESYARERSTVARIQFRLPDGSSFTNQFPSDAPLEEARQFAAQTVGNTYGNFSLATMFPRREFTKEDYKKKLLDLELAPSASVVLLPAGRPTASIVHSSSGDIWTLLGTVLYPFLAIWRLISNFLFSNPPPTQTSVRVTSSEPPNPASSSKSEKREPVRKRVLEKRGDDFKKEGKIYRLRTQDDGEDENNTWNGNSTQQM

相同性解析による交差性が予測される生物種
Full Name UBX domain protein 4
Calculated molecular weight 508 aa, 57 kDa
Observed molecular weight 64-68 kDa
GenBank accession numberBC035594
Gene Symbol UBXD2
Gene ID (NCBI) 23190
RRIDAB_10732809
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92575
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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