Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells, PC-12 cells, HEK-293 cells, Jurkat cells, Neuro-2a cells |
| Positive IHC detected in | mouse testis tissue, mouse brain tissue, rat testis tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
66937-1-Ig targets UBR5 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, ae. aegypti |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18753 Product name: Recombinant human UBR5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2450-2799 aa of BC137234 Sequence: IAHGRENGADSILDLGLVDSSEKVQQENRKRHGSSRSVVDMDLDDTDDGDDNAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADAVFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin protein ligase E3 component n-recognin 5 |
| Calculated molecular weight | 2799 aa, 309 kDa |
| Observed molecular weight | 309 kDa |
| GenBank accession number | BC137234 |
| Gene Symbol | UBR5 |
| Gene ID (NCBI) | 51366 |
| RRID | AB_2882261 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O95071 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and >300 kDa protein with widespread expression in various cell types.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBR5 antibody 66937-1-Ig | Download protocol |
| WB protocol for UBR5 antibody 66937-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.
| ||
J Virol Multiple E3 ligases act as antiviral factors against SARS-CoV-2 via inducing the ubiquitination and degradation of ORF9b
| ||
Exp Eye Res Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.
| ||
Cell Death Dis CSN6 promotes melanoma proliferation and metastasis by controlling the UBR5-mediated ubiquitination and degradation of CDK9.
| ||
Adv Sci (Weinh) Phosphorylated SHMT2 Regulates Oncogenesis Through m6 A Modification in Lung Adenocarcinoma | ||
J Agric Food Chem Methionine and Leucine Promote mTOR Gene Transcription and Milk Synthesis in Mammary Epithelial Cells through the eEF1Bα-UBR5-ARID1A Signaling
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