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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBR4 Polyclonal antibody (15966-1-AP)

    WB analysis using 15966-1-AP (same clone as 15966-1-PBS)

    Various lysates were subjected to Tris-acetate gel system followed by western blot with 15966-1-AP (UBR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using UBR4 Polyclonal antibody (15966-1-AP)

    IP experiment of HeLa using 15966-1-AP (same clone as 15966-1-PBS)

    IP result of anti-UBR4 (IP:15966-1-AP, 4ug; Detection:15966-1-AP 1:2000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of human pancreas cancer using 15966-1-AP (same clone as 15966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of human pancreas cancer using 15966-1-AP (same clone as 15966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of human lung cancer using 15966-1-AP (same clone as 15966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of human lung cancer using 15966-1-AP (same clone as 15966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of mouse testis using 15966-1-AP (same clone as 15966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBR4 Polyclonal antibody (15966-1-AP)

    IHC staining of mouse testis using 15966-1-AP (same clone as 15966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15966-1-AP (UBR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBR4 Polyclonal antibody (15966-1-AP)

    IF Staining of HeLa using 15966-1-AP (same clone as 15966-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using UBR4 antibody (15966-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15966-1-PBS in a different storage buffer formulation.

UBR4 Polyclonal antibody, PBS Only

UBR4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15966-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15966-1-PBS targets UBR4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8740

Product name: Recombinant human UBR4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 451-799 aa of BC018694

Sequence: GLTCCICREGYKFQPTKVLGIYTFTKRVALEELENKPRKQQGYSTVSHFNIVHYDCHLAAVRLARGREEWESAALQNANTKCNGLLPVWGPHVPESAFATCLARHNTYLQECTGQREPTYQLNIHDIKLLFLRFAMEQSFSADTGGGGRESNIHLIPYIIHTVLYVLNTTRATSREEKNLQGFLEQPKEKWVESAFEVDGPYYFTVLALHILPPEQWRATRVEILRRLLVTSQARAVAPGGATRLTDKAVKDYSAYRSSLLFWALVDLIYNMFKKVPTSNTEGGWSCSLAEYIRHNDMPIYEAADKALKTFQEEFMPVETFSEFLDVAGLLSEITDPESFLKDLLNSVP

相同性解析による交差性が予測される生物種
Full Name ubiquitin protein ligase E3 component n-recognin 4
Calculated molecular weight 5183 aa, 570 kDa
Observed molecular weight570 kDa, 270 kDa
GenBank accession numberBC018694
Gene Symbol UBR4
Gene ID (NCBI) 23352
RRIDAB_2878202
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T4S7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UBR4 (ubiquitin N-recognin domain-containing E3 ligase 4), also named P600, is an E3 ligase that can bind N-end rule substrates. UBR4-deficient mice die during embryogenesis and exhibit pleiotropic abnormalities (PMID: 23431188). UBR4 plays a role in membrane morphogenesis and extracellular matrix-mediated cell survival and signaling, human papillomavirus (HPV) E7-mediated cellular immortalization, and migration and development of neurons (PMID: 33658508, 25084275). The full-length UBR4 protein has a predicted size of ~570 kDa.

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