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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using UBQLN3 Polyclonal antibody (23372-1-AP)

    WB analysis of mouse testis using 23372-1-AP (same clone as 23372-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23372-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using UBQLN3 Polyclonal antibody (23372-1-AP)

    WB analysis of HepG2 using 23372-1-AP (same clone as 23372-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23372-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using UBQLN3 Polyclonal antibody (23372-1-AP)

    WB analysis of human testis using 23372-1-AP (same clone as 23372-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23372-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using UBQLN3 Polyclonal antibody (23372-1-AP)

    IHC staining of human testis using 23372-1-AP (same clone as 23372-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23372-1-AP (UBQLN3 antibody at dilution of 1:50. This data was developed using the same antibody clone with 23372-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using UBQLN3 Polyclonal antibody (23372-1-AP)

    IHC staining of human testis using 23372-1-AP (same clone as 23372-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23372-1-AP (UBQLN3 antibody at dilution of 1:50. This data was developed using the same antibody clone with 23372-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UBQLN3 Polyclonal antibody (23372-1-AP)

    IF Staining of HepG2 using 23372-1-AP (same clone as 23372-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UBQLN3 antibody (23372-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23372-1-PBS in a different storage buffer formulation.

UBQLN3 Polyclonal antibody, PBS Only

UBQLN3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23372-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

UBQLN 3, Ubiquilin-3, ubiquilin3, ubiquilin 3, TUP1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23372-1-PBS targets UBQLN3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19982

Product name: Recombinant human Ubiquilin 3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 306-620 aa of BC036743

Sequence: WTSTHGGSGSRQGRQDGDQDAPDIRNRFPNFLGIIRLYDYLQQLHENPQSLGTYLQGTASALSQSQEPPPSVNRVPPSSPSSQEPGSGQPLPEESVAIKGRSSCPAFLRYPTENSTGQGGDQDGAGKSSTGHSTNLPDLVSGLGDSANRVPFAPLSFSPTAAIPGIPEPPWLPSPAYPRSLRPDGMNPAPQLQDEIQPQLPLLMHLQAAMANPRALQALRQIEQGLQVLATEAPRLLLWFMPCLAGTGSVAGGIESREDPLMSEDPLPNPPPEVFPALDSAELGFLSPPFLHMLQDLVSTNPQQLQPEAHFQVQL

相同性解析による交差性が予測される生物種
Full Name ubiquilin 3
Calculated molecular weight 655 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC036743
Gene Symbol UBQLN3
Gene ID (NCBI) 50613
RRIDAB_2879268
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H347
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UBQLN3(Ubiquilin-3) may interact with the testis-specific cyclin A1 and regulate cell cycle progression during spermatogenesis, as does frog Xdrp1, with which UBQLN3 shares sequence similarity(PMID:10831842).UBQLN3 expression is not observed in mouse eye, thyroid, pancreas,smooth muscle, ovary, submaxillary gland, uterus, and epididymis by northern blot(PMID:10831842 ).

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