Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
13568-1-AP targets UBQLN3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4462 Product name: Recombinant human Ubiquilin 3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 305-655 aa of BC036743 Sequence: PWTSTHGGSGSRQGRQDGDQDAPDIRNRFPNFLGIIRLYDYLQQLHENPQSLGTYLQGTASALSQSQEPPPSVNRVPPSSPSSQEPGSGQPLPEESVAIKGRSSCPAFLRYPTENSTGQGGDQDGAGKSSTGHSTNLPDLVSGLGDSANRVPFAPLSFSPTAAIPGIPEPPWLPSPAYPRSLRPDGMNPAPQLQDEIQPQLPLLMHLQAAMANPRALQALRQIEQGLQVLATEAPRLLLWFMPCLAGTGSVAGGIESREDPLMSEDPLPNPPPEVFPALDSAELGFLSPPFLHMLQDLVSTNPQQLQPEAHFQVQLEQLRSMGFLNREANLQALIATGGDVDAAVEKLRQS 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquilin 3 |
| Calculated molecular weight | 655 aa, 71 kDa |
| Observed molecular weight | 71 kDa |
| GenBank accession number | BC036743 |
| Gene Symbol | UBQLN3 |
| Gene ID (NCBI) | 50613 |
| RRID | AB_2241124 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H347 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBQLN3(Ubiquilin-3) may interact with the testis-specific cyclin A1 and regulate cell cycle progression during spermatogenesis, as does frog Xdrp1, with which UBQLN3 shares sequence similarity(PMID:10831842).UBQLN3 expression is not observed in mouse eye, thyroid, pancreas,smooth muscle, ovary, submaxillary gland, uterus, and epididymis by northern blot(PMID:10831842 ).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for UBQLN3 antibody 13568-1-AP | Download protocol |
| WB protocol for UBQLN3 antibody 13568-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Fertil Steril Potential biomarkers of nonobstructive azoospermia identified in microarray gene expression analysis. | ||
Mol Reprod Dev Ubqln3, a testis-specific gene, is dispensable for embryonic development and spermatogenesis in mice. |
