Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, LNCaP cells, K-562 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67318-1-Ig targets UBP1 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5010 Product name: Recombinant human UBP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 280-540 aa of BC047235 Sequence: IEDAVEHEQKKSSKRTLPADYGDSLAKRGSCSPWPDAPTAYVNNSPSPAPTFTSPQQSTCSVPDSNSSSPNHQGDGASQTSGEQIQPSATIQETQQWLLKNRFSSYTRLFSNFSGADLLKLTKEDLVQICGAADGIRLYNSLKSRSVRPRLTIYVCREQPSSTVLQGQQQAASSASENGSGAPYVYHAIYLEEMIASEVARKLALVFNIPLHQINQVYRQGPTGIHILVSDQMVQNFQDESCFLFSTVKAESSDGIHIILK 相同性解析による交差性が予測される生物種 |
| Full Name | upstream binding protein 1 (LBP-1a) |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 65-68 kDa |
| GenBank accession number | BC047235 |
| Gene Symbol | UBP1 |
| Gene ID (NCBI) | 7342 |
| RRID | AB_2882578 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NZI7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBP1, also known as Transcription factor LBP-1, is a 540 amino acid protein, which is expressed in adrenal tissue, JEG-3, NCI-H295A, Hep-G2 and HeLa cell lines. UBP1 localizes in nuclear and can be induced by HIV-1 infection of lymphocytes. UBP1 functions as a transcriptional activator in a promoter context-dependent manner and modulates the placental expression of CYP11A1. UBP1 is involved in regulation of the alpha-globin gene in erythroid cells and represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBP1 antibody 67318-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |