Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, human kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13856-1-AP targets UBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4830 Product name: Recombinant human UBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 280-540 aa of BC047235 Sequence: IEDAVEHEQKKSSKRTLPADYGDSLAKRGSCSPWPDAPTAYVNNSPSPAPTFTSPQQSTCSVPDSNSSSPNHQGDGASQTSGEQIQPSATIQETQQWLLKNRFSSYTRLFSNFSGADLLKLTKEDLVQICGAADGIRLYNSLKSRSVRPRLTIYVCREQPSSTVLQGQQQAASSASENGSGAPYVYHAIYLEEMIASEVARKLALVFNIPLHQINQVYRQGPTGIHILVSDQMVQNFQDESCFLFSTVKAESSDGIHIILK 相同性解析による交差性が予測される生物種 |
| Full Name | upstream binding protein 1 (LBP-1a) |
| Calculated molecular weight | 60 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC047235 |
| Gene Symbol | UBP1 |
| Gene ID (NCBI) | 7342 |
| RRID | AB_2304131 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZI7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBP1, also known as Transcription factor LBP-1, is a 540 amino acid protein, which is expressed in adrenal tissue, JEG-3, NCI-H295A, Hep-G2 and HeLa cell lines. UBP1 localizes in nuclear and can be induced by HIV-1 infection of lymphocytes. UBP1 functions as a transcriptional activator in a promoter context-dependent manner and modulates the placental expression of CYP11A1. UBP1 is involved in regulation of the alpha-globin gene in erythroid cells and represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBP1 antibody 13856-1-AP | Download protocol |
| WB protocol for UBP1 antibody 13856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
