Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 14253-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HUVEC using 14253-1-AP
HUVEC cells were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14253-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 14253-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14253-1-AP (UBL4A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 14253-1-AP
IP result of anti-UBL4A (IP:14253-1-AP, 4ug; Detection:14253-1-AP 1:500) with mouse testis tissue lysate 4400ug.
IHC staining of human ovary tumor using 14253-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14253-1-AP (UBL4A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary tumor using 14253-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14253-1-AP (UBL4A antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 14253-1-AP
Immunofluorescent analysis of HepG2 cells, using UBL4A antibody 14253-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Featured Product
KD/KO Validated

UBL4A Polyclonal antibody

UBL4A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14253-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Ubiquitin-like protein 4A, ubiquitin like 4A, GDX, G6PD, DXS254E

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HUVEC cells, mouse testis tissue, human brain tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 13 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

14253-1-AP targets UBL4A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5526 Product name: Recombinant human UBL4A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-157 aa of BC043346 Sequence: MQLTVKALQGRECSLQVPEDELVSTLKQLVSEKLNVPVRQQRLLFKGKALADGKRLSDYSIGPNSKLNLVVKPLEKVLLEEGEAQRLADSPPPQVWQLISKVLARHFSAADASRVLEQLQRDYERSLSRLTLDDIERLASRFLHPEVTETMEKGFSK 相同性解析による交差性が予測される生物種
Full Name	ubiquitin-like 4A
Calculated molecular weight	18 kDa
Observed molecular weight	17 kDa
GenBank accession number	BC043346
Gene Symbol	UBL4A
Gene ID (NCBI)	8266
RRID	AB_2288205
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11441
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for UBL4A antibody 14253-1-AP	Download protocol
IHC protocol for UBL4A antibody 14253-1-AP	Download protocol
IP protocol for UBL4A antibody 14253-1-AP	Download protocol
WB protocol for UBL4A antibody 14253-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Clin Invest Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer Authors - Xiaochao Tan View Article
mouse	WB,IF	J Immunol UBL4A Augments Innate Immunity by Promoting the K63-Linked Ubiquitination of TRAF6. Authors - Shu-Jie Peng KD Validated View Article
human	WB	J Virol SGTA-dependent regulation of Hsc70 promotes cytosol entry of SV40 from the ER. Authors - Allison Dupzyk View Article
pig	WB	J Nutr Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs. Authors - Agus Suryawan View Article
human	WB	J Inherit Metab Dis Mutations in GET4 disrupt the transmembrane domain recognition complex pathway. Authors - Mitali A Tambe View Article
pig	WB	Pediatr Res Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets Authors - Agus Suryawan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 14253-1-AP

WB analysis of HUVEC using 14253-1-AP

WB analysis of mouse testis using 14253-1-AP

WB analysis of human brain using 14253-1-AP

IP experiment of mouse testis using 14253-1-AP

IHC staining of human ovary tumor using 14253-1-AP

IHC staining of human ovary tumor using 14253-1-AP

IF Staining of HepG2 using 14253-1-AP

UBL4A Polyclonal antibody

UBL4A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer

UBL4A Augments Innate Immunity by Promoting the K63-Linked Ubiquitination of TRAF6.

SGTA-dependent regulation of Hsc70 promotes cytosol entry of SV40 from the ER.

Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.

Mutations in GET4 disrupt the transmembrane domain recognition complex pathway.

Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets