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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBFD1 Polyclonal antibody (32161-1-AP)

    WB analysis using 32161-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32161-1-AP (UBFD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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UBFD1 Polyclonal antibody

UBFD1 Polyclonal Antibody for WB, ELISA
Cat No. 32161-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Ubiquitin-binding protein homolog, Ubiquitin Family Domain Containing 1 , Ubiquitin domain-containing protein UBFD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32161-1-AP targets UBFD1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBFD1 fusion protein Ag36361 相同性解析による交差性が予測される生物種
Full Name ubiquitin family domain containing 1
Calculated molecular weight309aa, 33 kDa
Observed molecular weight40 kDa
GenBank accession numberNM_019116
Gene Symbol UBFD1
Gene ID (NCBI) 56061
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO14562
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBFD1 (Ubiquitin Family Domain Containing 1) is a polyubiquitin-binding protein containing ubiquitin-like structural domains that are widely involved in protein degradation and cell signaling. This protein shows aberrant expression in a variety of cancers, especially in estrogen receptor (ER)-positive breast cancer, where high expression of UBFD1 is closely associated with poor prognosis of patients, and is considered as a potential prognostic biomarker and therapeutic target. In addition, UBFD1 was also found to be associated with MYC-mediated tumor aggressiveness in T-cell acute lymphoblastic leukemia (T-ALL), which functionally involves the endoplasmic reticulum-associated degradation (ERAD) pathway. UBFD1 is also expressed in inner ear hair cells and ear epithelial cells, suggesting that it may play a role in hearing-related diseases.

Protocols

Product Specific Protocols
WB protocol for UBFD1 antibody 32161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols