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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using UBE4A Polyclonal antibody (21548-1-AP)

    WB analysis of HEK-293 using 21548-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using UBE4A Polyclonal antibody (21548-1-AP)

    WB analysis of mouse brain using 21548-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using UBE4A Polyclonal antibody (21548-1-AP)

    WB analysis of L02 using 21548-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using UBE4A Polyclonal antibody (21548-1-AP)

    WB analysis of Jurkat using 21548-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using UBE4A Polyclonal antibody (21548-1-AP)

    WB analysis of HepG2 using 21548-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using UBE4A Polyclonal antibody (21548-1-AP)

    IP experiment of mouse brain using 21548-1-AP

    IP result of anti-UBE4A (IP:21548-1-AP, 4ug; Detection:21548-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  • Featured Product
  • KD/KO Validated

UBE4A Polyclonal antibody

UBE4A Polyclonal Antibody for WB, IP, ELISA
Cat No. 21548-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

E4, KIAA0126, UBE4A, UBOX2, UFD2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, L02 cells, Jurkat cells, HepG2 cells
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21548-1-AP targets UBE4A in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE4A fusion protein Ag16211 相同性解析による交差性が予測される生物種
Full Name ubiquitination factor E4A (UFD2 homolog, yeast)
Calculated molecular weight 1073 aa, 124 kDa
Observed molecular weight 123 kDa
GenBank accession numberBC112367
Gene Symbol UBE4A
Gene ID (NCBI) 9354
RRIDAB_10755303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14139
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBE4A antibody 21548-1-APDownload protocol
IP protocol for UBE4A antibody 21548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.

Authors - Xianyan Hu
View Article
humanWB

Biochim Biophys Acta Gene Regul Mech

UBE4A catalyzes NRF1 ubiquitination and facilitates DDI2-mediated NRF1 cleavage

Authors - Xianyan Hu
  • KD Validated
View Article