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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MMS2 Polyclonal antibody

MMS2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10689-1-AP

Synonyms

DDVit 1, DDVIT1, EDAF 1, EDPF 1, EDPF1, MMS2, MMS2 homolog, UB2V2, UBE2V2, UEV 2, UEV2, Vitamin D3 inducible protein

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 10689-1-AP

    WB analysis of HEK-293 using 10689-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 10689-1-AP

    WB analysis of A375 using 10689-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10689-1-AP

    WB analysis of HeLa using 10689-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 10689-1-AP

    WB analysis of K-562 using 10689-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 10689-1-AP

    WB analysis of Jurkat using 10689-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10689-1-AP

    WB analysis of HeLa using 10689-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 10689-1-AP

    WB analysis of HL-60 using 10689-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 10689-1-AP

    WB analysis of rat liver using 10689-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10689-1-AP

    WB analysis of HEK-293 using 10689-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10689-1-AP

    WB analysis of HEK-293 using 10689-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 10689-1-AP

    WB analysis of K-562 using 10689-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 10689-1-AP

    WB analysis of human brain using 10689-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10689-1-AP (MMS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 10689-1-AP

    IHC staining of human colon cancer using 10689-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10689-1-AP (MMS2 antibody) at dilution of 1:100 (under 25x lens).

  • IHC staining of human colon using 10689-1-AP

    IHC staining of human colon using 10689-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10689-1-AP (MMS2 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 10689-1-AP

    IHC staining of human colon using 10689-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10689-1-AP (MMS2 antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 10689-1-AP

    IF Staining of HeLa using 10689-1-AP

    Immunofluorescent analysis of HeLa cells using 10689-1-AP (MMS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HEK-293 using 10689-1-AP

    IF Staining of HEK-293 using 10689-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10689-1-AP (MMS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, A375 cells, HeLa cells, HL-60 cells, human brain tissue, Jurkat cells, K-562 cells, rat liver tissue
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10689-1-AP targets MMS2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMS2 fusion protein Ag1111 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2 variant 2
Calculated molecular weight 16 kDa
Observed molecular weight 16-20 kDa
GenBank accession numberBC007051
Gene symbol UBE2V2
Gene ID (NCBI) 7336
RRIDAB_2211656
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MMS2 antibody 10689-1-APDownload protocol
IHC protocol for MMS2 antibody 10689-1-APDownload protocol
IF protocol for MMS2 antibody 10689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF,WB

Transl Stroke Res

MicroRNA-193a-5p Rescues Ischemic Cerebral Injury by Restoring N2-Like Neutrophil Subsets

Authors - Ziping Han
View Article
humanWB, IF

Biochim Biophys Acta

Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.

Authors - Fan Chai
View Article