Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66897-1-Ig targets UBE2S in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5378 Product name: Recombinant human UBE2S protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-222 aa of BC066948 Sequence: MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHLNVGANGEICVNVLKRDWTAELGIRHVLLTIRCLLIHPNPESALNEEAGRLLLENSEEYAARARLLTEIHGGAGGPSGRAEAGRALASGTAASSTDPGAPGGLGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRRL 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin-conjugating enzyme E2S |
| Calculated molecular weight | 222 aa, 24 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC066948 |
| Gene Symbol | UBE2S |
| Gene ID (NCBI) | 27338 |
| RRID | AB_2882226 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16763 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for UBE2S antibody 66897-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |