Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of Jurkat using 14077-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14077-1-AP (UBE2R2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 14077-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14077-1-AP (UBE2R2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14077-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14077-1-AP (UBE2R2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human spleen using 14077-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14077-1-AP (UBE2R2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human spleen using 14077-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14077-1-AP (UBE2R2 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 14077-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14077-1-AP (UBE2R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

UBE2R2 Polyclonal antibody

UBE2R2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14077-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ubiquitin protein ligase R2, Ubiquitin carrier protein R2, UBC3B, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme R2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, mouse spleen tissue
Positive IHC detected in	human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below

Product Information

14077-1-AP targets UBE2R2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5202 Product name: Recombinant human UBE2R2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-238 aa of BC047584 Sequence: MAQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDLYDDDIDDEDEEEEDADCYDDDDSGNEES 相同性解析による交差性が予測される生物種
Full Name	ubiquitin-conjugating enzyme E2R 2
Calculated molecular weight	27 kDa
Observed molecular weight	28-30 kDa
GenBank accession number	BC047584
Gene Symbol	UBE2R2
Gene ID (NCBI)	54926
RRID	AB_2878010
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q712K3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for UBE2R2 antibody 14077-1-AP	Download protocol
IHC protocol for UBE2R2 antibody 14077-1-AP	Download protocol
WB protocol for UBE2R2 antibody 14077-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	EMBO J Shared and distinct mechanisms of UBA1 inactivation across different diseases Authors - Jason C Collins View Article
mouse	WB	JCI Insight PMID: 31292296 Authors - Isabelle K Vila View Article
human	WB	bioRxiv Shared and Distinct Mechanisms of UBA1 Inactivation Across Different Diseases Authors - Jason C Collins View Article
	WB	Nat Commun BiDAC-dependent degradation of plasma membrane proteins by the endolysosomal system Authors - Sammy Villa View Article
human	WB	Exp Gerontol p53 accelerates endothelial cell senescence in diabetic retinopathy by enhancing FoxO3a ubiquitylation and degradation via UBE2L6 Authors - Ying Cheng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 14077-1-AP

WB analysis of mouse spleen using 14077-1-AP

WB analysis of HeLa using 14077-1-AP

IHC staining of human spleen using 14077-1-AP

IHC staining of human spleen using 14077-1-AP

IF Staining of HeLa using 14077-1-AP

UBE2R2 Polyclonal antibody

UBE2R2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Shared and distinct mechanisms of UBA1 inactivation across different diseases

PMID: 31292296

Shared and Distinct Mechanisms of UBA1 Inactivation Across Different Diseases

BiDAC-dependent degradation of plasma membrane proteins by the endolysosomal system

p53 accelerates endothelial cell senescence in diabetic retinopathy by enhancing FoxO3a ubiquitylation and degradation via UBE2L6