• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using UBE2O Recombinant monoclonal antibody (87282-1-RR)

    WB analysis using 87282-1-RR (same clone as 87282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87282-1-RR (UBE2O antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87282-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using UBE2O Recombinant monoclonal antibody (87282-1-RR)

    WB analysis using 87282-1-RR (same clone as 87282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87282-1-RR (UBE2O antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87282-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using UBE2O Recombinant monoclonal antibody (87282-1-RR)

    IF Staining of A431 using 87282-1-RR (same clone as 87282-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using UBE2O antibody (87282-1-RR, Clone: 252545E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87282-1-PBS in a different storage buffer formulation.

  • Affinity of 87282-1-RR

    Affinity of 87282-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87282-1-RR against Human UBE2O were performed. The affinity constant is 16.1 nM.

UBE2O Recombinant monoclonal antibody, PBS Only

UBE2O Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87282-1-PBS
Clone No.252545E2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

(E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, E2-230K, EC:2.3.2.24, KIAA1734

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87282-1-PBS targets UBE2O in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8580

Product name: Recombinant human UBE2O protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-343 aa of BC007822

Sequence: SSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRL

相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2O
Calculated molecular weight 1292 aa, 141 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC007822
Gene Symbol UBE2O
Gene ID (NCBI) 63893
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9C0C9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UBE2O, also named as KIAA1734, belongs to the ubiquitin-conjugating enzyme family. It catalyzes the covalent attachment of ubiquitin to other proteins. UBE2O has a calculated molecular mass of 141 kDa, and many bands can be detected as 140-170 kDa and 200-230 kDa (PMID: 28774900, 23455153, 28774922, 11311559).

||
New chat

Able™

正在加载，请稍候...