Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, mouse testis tissue, human brain tissue, rat brain tissue, rat testis tissue |
| Positive IHC detected in | human gliomas tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
11834-3-AP targets UBE2K in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2415 Product name: Recombinant human UBE2K protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC022804 Sequence: MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) |
| Calculated molecular weight | 200 aa, 22 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC022804 |
| Gene Symbol | UBE2K |
| Gene ID (NCBI) | 3093 |
| RRID | AB_2241168 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61086 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBE2K antibody 11834-3-AP | Download protocol |
| WB protocol for UBE2K antibody 11834-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Oncol UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma | ||
Biochem Biophys Res Commun Ixazomib inhibits myeloma cell proliferation by targeting UBE2K. | ||
Biomed Environ Sci Ezrin Promotes the Proliferation, Migration, and Invasion of Ovarian Cancer Cells. | ||
Neuropathology Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation. |
