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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2G2 Polyclonal antibody (10722-1-AP)

    WB analysis using 10722-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10722-1-AP (UBE2G2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBE2G2 Polyclonal antibody (10722-1-AP)

    IHC staining of mouse testis using 10722-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10722-1-AP (UBE2G2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBE2G2 Polyclonal antibody

UBE2G2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10722-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

E2 ubiquitin-conjugating enzyme G2, EC:2.3.2.23, UBC 7, UBC7, UBE2G 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10722-1-AP targets UBE2G2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1082

Product name: Recombinant human UBE2G2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-165 aa of BC008351

Sequence: MAGTALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKSLGL

相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast)
Calculated molecular weight 19 kDa
Observed molecular weight 16-19 kDa
GenBank accession numberBC008351
Gene Symbol UBE2G2
Gene ID (NCBI) 7327
RRIDAB_2256726
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP60604
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBE2G2 antibody 10722-1-APDownload protocol
IHC protocol for UBE2G2 antibody 10722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols