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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2F Polyclonal antibody (17056-1-AP)

    WB analysis using 17056-1-AP (same clone as 17056-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using UBE2F Polyclonal antibody (17056-1-AP)

    WB analysis of Jurkat using 17056-1-AP (same clone as 17056-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using UBE2F Polyclonal antibody (17056-1-AP)

    WB analysis of mouse ovary using 17056-1-AP (same clone as 17056-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using UBE2F Polyclonal antibody (17056-1-AP)

    IHC staining of human skin using 17056-1-AP (same clone as 17056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using UBE2F Polyclonal antibody (17056-1-AP)

    IHC staining of human skin using 17056-1-AP (same clone as 17056-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using UBE2F Polyclonal antibody (17056-1-AP)

    IF Staining of NIH/3T3 using 17056-1-AP (same clone as 17056-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 17056-1-AP (UBE2F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

UBE2F Polyclonal antibody, PBS Only

UBE2F Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17056-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17056-1-PBS targets UBE2F in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10764

Product name: Recombinant human UBE2F protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-185 aa of BC010549

Sequence: MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQLTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIEAAEHHLRDKEDFRNKVDDYIKRYAR

相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2F (putative)
Calculated molecular weight 185 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC010549
Gene Symbol UBE2F
Gene ID (NCBI) 140739
RRIDAB_2210295
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969M7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UBE2F(Ubiquitin-conjugating enzyme E2 F) is also named as NCE2(NEDD8-conjugating enzyme 2) and belongs to the ubiquitin-conjugating enzyme family.The canonical uBE2M catalyses the modification of most cullins, whereas uBE2F is specifically responsible for cuL5 neddylation. uBE2F is regulated differently than uBE2M, supporting the idea that neddylation is used to fine-tune ubiquitin-chain formation by different cullin-dependent E3s(PMID:19465892).

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