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Validation Data Gallery

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  • Western Blot (WB) analysis of human thyroid gland tissue using UBE2E2 Polyclonal antibody (11844-1-AP)

    WB analysis of human thyroid gland using 11844-1-AP (same clone as 11844-1-PBS)

    human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using UBE2E2 Polyclonal antibody (11844-1-AP)

    WB analysis of human brain using 11844-1-AP (same clone as 11844-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse embryo tissue using UBE2E2 Polyclonal antibody (11844-1-AP)

    WB analysis of mouse embryo using 11844-1-AP (same clone as 11844-1-PBS)

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using UBE2E2 Polyclonal antibody (11844-1-AP)

    IHC staining of human gliomas using 11844-1-AP (same clone as 11844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11844-1-AP (UBE2E2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UBE2E2 Polyclonal antibody (11844-1-AP)

    IF Staining of HepG2 using 11844-1-AP (same clone as 11844-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using UBE2E2 antibody 11844-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

UBE2E2 Polyclonal antibody, PBS Only

UBE2E2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11844-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11844-1-PBS targets UBE2E2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2509

Product name: Recombinant human UBE2E2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-201 aa of BC022332

Sequence: MSTEAQRVDDSPSTSGGSSDGDQRESVQQEPEREQVQPKKKEGKISSKTAAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT

相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast)
Calculated molecular weight 201 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC022332
Gene Symbol UBE2E2
Gene ID (NCBI) 7325
RRIDAB_2210274
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LR5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UBE2E2(ubiquitin-conjugating enzyme E2 E2) is also named as UBCH8 and belongs to the ubiquitin-conjugating enzyme family. This gene encodes a 22 kDa protein that functions as a major ISG15 E2 enzyme raises the possibility that ISG15 conjugation might use UbcH8-competent Ub E3 enzymes and also function as an E2 for several Ub E3 enzymes, including both HECT E3s and RING E3s(PMID: 15131269).